JEONBUK NATIONAL UNIVERSITY
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Development of NAD(P)H Regenerative BioreactorDevelopment of NAD(P)H Regenerative Bioreactor Test Host Jung Eun-Sun, R. Nirmala, R. Jayabalan Test Purpose and Contents Test Purpose and Dehydrogenase is a very important enzyme acting as a catalyst of reaction to produce high value added chiral compound by revivifying substrate as NAD(P)+dependence, but is not used for industrial production.
It is caused by a problem which continues to recycle coenzyme continuously (NAD(P)+NAD(P)H) in reaction. The laboratory is performing researches to construct efficient process by coupling electrochemical recycling reaction to enzyme reaction. -
Efficient increase of two phase system enzyme reaction by application of Bicontinuous microemulsion
Efficient increase of two phase system enzyme reaction by application of Bicontinuous microemulsion Test Host R. Jayabalan Test Purpose and Contents When hydrophobic material is involved in enzyme reaction, it is not developed to industrial process of production since efficiency of reaction is very low, For, solubility of enzyme and reaction species makes a trouble
Strive to settle such a problem by applying Bicontinuous micremulsion in the medium of reaction Research on enzymatic synthesis reaction (creation of hexyl glucoside which is an emulsifying agent followed by combination between glucose by B-glucosidase and hexanol) and enzymatic hydrolysis reaction (production of (S)-ketoprofen which is non-steroidal analgesic inflammation drug through hydrolysis of (R.S)-ketoprofen ester by esterase). -
Development of Biosensor with oxidation reduction enzyme as bioreceptor
Development of Biosensor with oxidation reduction enzyme as bioreceptor Test Host Park Song-Yi, Kim Bo-Hee Test Purpose and Contents NAD(P)+dependence dehydrogenase is an important enzyme in production of material. Research to develop amperometric biosensor by coupling electrochemical oxidation reaction to NAD(P)+ of NAD(PH with enzymatic oxidation reaction is an important assignment in the world.
However, it is very difficult to fixate NAD(P)+ with dehydrogenase on electrode surface. Even if it is fixed, interaction between coenzyme and enzyme is damaged or there is a problem with slow electronic transport between electrode and coenzyme. Therefore, perform researches to construct amperometric biosensor with NAD(P)+dependent enzyme as bioreceptor by exploring a new mediator, utilizing carbon cloth or carbon nanotube as an electrode material to supporting electrode and applying a new fixation method.